NUMERO: #20240724

Título: ANALYSIS OF BRAF p.V600E MUTATION BY ddPCR IN PLASMA OF PATIENTS WITH AMELOBLASTOMA

Nome do Apresentador: Isadora Pereira GOMES

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Objective: To investigate whether tumor DNA could reach blood circulation and, consequently, if cell-free DNA (cfDNA) could be detected by liquid biopsy in the plasma of patients with ameloblastoma. Study design: Peripheral blood samples and tumor tissue were collected from five patients. Mutational status analysis for BRAF p.V600E mutation was performed on gDNA (tumor tissue) and cfDNA (plasma) using allele-specific real-time qPCR and droplet-digital PCR (ddPCR), respectively, in the selected cases. Results: BRAF p.V600E was detected in four (4/5) ameloblastoma tissue samples, while all tested ameloblastoma plasmas were negative for the detection of BRAF p.V600E mutation in cfDNA. Conclusion: These preliminary findings suggest that with the current technology, BRAF p. V6000E mutation cannot be detected in ameloblastoma patients' plasma. Financial support: CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico)/Brazil, FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais)/Brazil, CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)/Brazil and Brazilian Ministry of Health (MS-SCTIE-DECIT, Process 405941/2022-6).

Autor 1:  Isadora Pereira GOMES

E-mail 1:  [email protected]

Autor 2:   Lucyene Miguita LUIZ

E-mail 2:  [email protected]

Autor 3 :  Bruna Pizziolo COURA

E-mail 3:  [email protected]

Autor 4:  Wagner Henriques de CASTRO

E-mail 4:  [email protected]

Autor 5:  Ricardo Santiago GOMEZ

E-mail 5:  [email protected]

Autor 6:  Luciana BASTOS-RODRIGUES

E-mail 6:  [email protected]

Autor 7:  Carolina Cavalieri GOMES

E-mail 7:  [email protected]