NUMERO: #20240711

Título: EXPRESSION OF SARS-CoV-2 CELLULAR ENTRY FACTORS IN PLEOMORPHIC ADENOMA

Nome do Apresentador: Fernanda Aragão FELIX

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Aim: to assess the gene expression of SARS-CoV-2 cellular entry factors ACE2, TMPRSS2 and FURIN in pleomorphic adenoma in comparison to normal salivary glands. Methods: Twenty-five formalin-fixed paraffin embedded samples were obtained, consisting of 15 pleomorphic adenoma, and 10 normal salivary glands. RNA extraction and RT-qPCR were performed to assess mRNA expression of ACE2, TMPRSS2 and FURIN. The results of target transcripts were normalized to endogenous levels. Results: mRNA expressions of ACE2, TMPRSS2 and FURIN were higher in normal salivary glands compared to the levels in pleomorphic adenoma (p<0.05). A positive correlation was observed between ACE2, TMPRSS2, and FURIN in pleomorphic adenoma (p<0.05). Conclusion: SARS-CoV-2 cellular entry factors are expressed in salivary glands. Our analysis revealed higher levels in normal glands when compared to the expression in salivary glands affected by a salivary gland tumor. Pleomorphic adenoma may downregulate the expression SARS-CoV-2 entry factors in the salivary glands analyzed. SUPPORT: FAPEMIG, CAPES

Autor 1:  Fernanda Aragão FELIX

E-mail 1:  [email protected]

Autor 2:   Flávia Martins Vasconcellos FILLIÚ

E-mail 2:  [email protected]

Autor 3 :  Marina Gonçalves DINIZ

E-mail 3:  [email protected]

Autor 4:  Silvia Ferreira de SOUSA

E-mail 4:  [email protected]