NUMERO: #20240608

Título: COLD ATMOSPHERIC PRESSURE PLASMA MAY PREVENT ORAL MUCOSITIS-RELATED CANDIDEMIA IN IMMUNOSUPPRESSIVE RATS

Nome do Apresentador: Noala Vicensoto Moreira MILHAN

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Áreas afins no caráter multidisciplinar

Resumo: Objective: To investigate the effectiveness of cold atmospheric pressure plasma (CAP) in preventing oral mucositis-related candidemia in immunosuppressive rats once oral mucositis may be a gateway to hematogenic dissemination of Candida albicans. Study design: Forty Wistar rats were divided into control and CAP groups. One cycle of chemotherapy with cisplatin and four with 5-fluorouracil were performed, with oral inoculation of C. albicans on the 2nd, 3rd and 5th days. Oral mucositis was delimited in lateral border of tongue with acetic acid on the 4th day, followed by CAP treatment for 5 min on 4th and 5th days. The euthanasia was performed 1 and 2 days after the last treatment, after which the tongue, lung, liver, kidney and spleen were processed for fungal counting on specific agar. Body weight was assessed daily and complete blood analysis was conducted before euthanasia. Results: There was loss of body weight and reduction in the white blood cells confirming the immunosuppression. CAP decreased the fungal counting from the tongue after 24 h (p<0.05). Interestingly, systemic fungal dissemination was also reduced after 24 and 72 h (p<0.05). Conclusion: CAP is a promising preventive approach for patients with oral mucositis at risk of candidemia.

Funding by FAPESP (São Paulo Research Foundation, 2019/25652-7; 2019/05856-7 and 2021/00046-7).

Autor 1:  Aline da Graça SAMPAIO

E-mail 1:  [email protected]

Autor 2:   Noala Vicensoto Moreira MILHAN

E-mail 2:  [email protected]

Autor 3 :  Fellype DO NASCIMENTO

E-mail 3:  [email protected]

Autor 4:  Konstantin Georgiev KOSTOV

E-mail 4:  [email protected]

Autor 5:  Cristiane Yumi KOGA-ITO

E-mail 5:  [email protected]