Título: GSK-3 ENZYME AS A PROMISING TARGET STRATEGY FOR TISSUE ENGINEERING
Nome do Apresentador: Iara Gonçalves AQUINO
Categoria do Trabalho: Painel de pesquisa científica (PPC)
Área Temática: Patologia Oral
Resumo: Objective: Cysts and tumors, periodontal diseases, and traumas can cause bone defects in the oral maxillofacial region. Tissue engineering has been widely researched to make the tissue regeneration process more predictable. Therefore, we evaluated the effects of pharmacological inhibition of the enzyme glycogen synthase kinase-3 (GSK-3) by Tideglusib on promoting biomineralization.Study design: OCCM-30 cementoblast cells were treated with Tideglusib at concentrations of 50, 100, and 200 nM, and evaluated for cell viability, alkaline phosphatase (ALP) activity, and formation of mineral nodules by alizarin red staining. The expression of Axin 2, osteopontin (Opn), bone sialoprotein (Bsp), ALP, osteocalcin (Ocn), and osterix (Osx) genes was measured by RT-PCR.Results: Cell viability was significantly reduced with treatment at 200 nM Tideglusib after 6 days. Tideglusib significantly increased ALP activity and mineral nodule formation with and without osteogenic induction at 8 and 14 days, respectively. Finally, Tideglusib also increased mRNA levels for Axin-2, as well as for the Opn, Bsp, Alp, Ocn, and Osx genes.Conclusion: Our results may contribute to a better understanding of the cellular and molecular events involved in the mineralization of bone tissue, improving the development of new biomaterials for bone regeneration procedures.
Autor 1: Iara Gonçalves AQUINO
E-mail 1: [email protected]
Autor 2: Arua Anaruma DAGNONE
E-mail 2: [email protected]
Autor 3 : Lucas Novaes TEIXEIRA
E-mail 3: [email protected]
Autor 4: Ana Luiza MILLÁS
E-mail 4: [email protected]
Autor 5: Paulo Noronha LISBOA-FILHO
E-mail 5: [email protected]
Autor 6: Francisco Humberto NOCITI JUNIOR
E-mail 6: [email protected]
Autor 7: Henrique Ballassini ABDALLA
E-mail 7: [email protected]
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