NUMERO: #20240129

Título: Organic depletion of salivary proteins after head and neck radiotherapy: preliminary results

Nome do Apresentador: Natália Rangel PALMIER

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Oncologia

Resumo: Objectives: To evaluate salivary protein quantification before, at the conclusion, and one year following head and neck radiotherapy (HNRT) for oral and oropharyngeal squamous cell carcinoma (OOSCC).
Study design: Salivary flow rates and protein estimation using the Bradford assay were performed. 10 µg of total protein was used as standard for comparison. Statistical analysis encompassed the Shapiro-Wilk and Friedman tests followed by Wilcoxon tests with Bonferroni correction.
Results: Significant decline in salivary flow at the end of RT (1.9 mL ± 1.3, p=0.011) and one year after RT (0.8 mL ± 0.9, p=0.002) compared to pre-HNRT (3.8 mL ± 2.2) and between the end of HNRT and one year after HNRT (p=0.001) was observed. Increased saliva required to obtain 10 µg of protein (p=0.001) was noted and supported by higher saliva volume required at the end of HNRT (47.6µL ± 42.2, p=0.003) and one year post-HNRT (63.2 µL ± 36.2, p=0.003) compared to pre-HNRT (15.7 µL ± 16.4).
Conclusions: Non-stimulated salivary flow and protein content at the end and one-year post-HNRT were significantly impaired. Reduction in the total amount of salivary proteins may be regarded as an important factor to be considered in future studies regarding salivary proteomic analysis post-HNRT.

Autor 1:  Natália Rangel PALMIER

E-mail 1:  [email protected]

Autor 2:   Iara Gonçalves AQUINO

E-mail 2:  [email protected]

Autor 3 :  Erison Santana Dos SANTOS

E-mail 3:  [email protected]

Autor 4:  Karina MORAIS-FARIA

E-mail 4:  [email protected]

Autor 5:  Thaís Bianca BRANDÃO

E-mail 5:  [email protected]

Autor 6:  Alan Roger SANTOS-SILVA

E-mail 6:  [email protected]

Autor 7:  Ana Carolina PRADO-RIBEIRO

E-mail 7:  [email protected]