NUMERO: #20240014

Título: NEUTROPHIL EXTRACELLULAR TRAPS IN OROPHARYNGEAL CANCER: DIFFERENT EXPRESSION ACCORDING TO THE HPV STATUS

Nome do Apresentador: Natália Santos BARCELOS

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Objective: Oropharyngeal squamous cell carcinoma (OP-SCC) represents a public health problem, and human papillomavirus (HPV) is one of the risk factors. Neutrophil extracellular traps (NETs) are meshes of DNA decorated with granular proteins. NETs have been identified in diverse cancers, whether associated with viruses or not. However, there is no information on NETs in OP-SCC. We aimed to evaluate the NETs release by neutrophils in the OP-SCC microenvironment, stratified by HPV status. Study design: This cross-sectional study analyzed OP-SCC biopsy specimens diagnosed from 1997 to 2021. HPV status was determined by p16 immunohistochemistry and "in situ" hybridization. Neutrophils were detected by CD66b immunohistochemistry, and immunofluorescence was used to identify NETs by co-localization of myeloperoxidase (MPO) and citrullinated histone H3 (H3Cit). Bivariate statistics was performed. A p-value of less than 0.05 was considered significant. Results: HPV-positive and HPV-negative OP-SCC had similar CD66b+ neutrophil infiltration (p>0.05), but the release of NETs was significantly increased in HPV-positive OP-SCC compared to HPV-negative OP-SCC (p<0.05). Conclusions: The presence of HPV may stimulate NET release in the OP-SCC microenvironment. Support: CAPES, FAPEMIG #APQ-01361-22.

Autor 1:  Natália Santos BARCELOS

E-mail 1:  [email protected]

Autor 2:   Adriana Aparecida Silva da COSTA

E-mail 2:  [email protected]

Autor 3 :  Tarcília Aparecida da SILVA

E-mail 3:  [email protected]

Autor 4:  Jorge Esquiche LEON

E-mail 4:  [email protected]

Autor 5:  Sergio Vitorino CARDOSO

E-mail 5:  [email protected]

Autor 6:  Maria Cássia Ferreira de AGUIAR

E-mail 6:  [email protected]

Autor 7:  Patrícia Carlos CALDEIRA

E-mail 7:  [email protected]