NUMERO: #20240005

Título: GENERATION AND CHARACTERIZATION OF CISPLATIN-RESISTANT ORAL CANCER CELL LINES DISPLAYING AN EMT SIGNATURE

Nome do Apresentador: Everton Freitas de MORAIS

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Oncologia

Resumo: Objectives: The present study aimed to establish two new cisplatin-resistant oral squamous cell carcinoma (OSCC) cell lines and investigate their biological characteristics.
Study Design: OSCC cell lines SCC-9 and HSC-3 were exposed to cisplatin using stepwise dose escalation methods, and a total of two cisplatin-resistant sublines were generated, after determination of 50% inhibitory concentration (IC50). The cisplatin-resistant cells were subjected to morphological analysis, migration and invasion assays and evaluation of epithelial-mesenchymal transition (EMT)-related phenotypes.
Results: The results demonstrated a significant increase in resistance to cisplatin-induced cell death in chemoresistant cell lines, which was accompanied by the acquisition of a fusiform shape and increased potential of migration and invasion. Furthermore, a significant increase in the expression of EMT markers and MMP-2 and -9 activities were observed.
Conclusions: This study provides novel insights into OSCC cisplatin resistance, providing a valuable tool for investigating the molecular pathways and putative markers of EMT that may be associated with this resistance phenotype in oral cancer.
Funding: FAPESP 2022/00994-5 and 2022/08908-0.

Autor 1:  Everton Freitas de MORAIS

E-mail 1:  [email protected]

Autor 2:   Rafaela Fernanda de SOUZA

E-mail 2:  [email protected]

Autor 3 :  Lilianny Querino Rocha de OLIVEIRA

E-mail 3:  [email protected]

Autor 4:  Fábio Haach TÉO

E-mail 4:  [email protected]

Autor 5:  Camila de Oliveira Rodini PEGORARO

E-mail 5:  [email protected]

Autor 6:  Ricardo Della COLETTA

E-mail 6:  [email protected]