NUMERO: #20220209

Título: DEPLETION OF MUCOEPIDERMOID CARCINOMA CANCER STEM CELLS BY HISTONE DEACETYLASE AND NFkB INHIBITORS

Nome do Apresentador: Paulo Victor Mendes PENAFORT

Categoria do Trabalho: Apresentação Oral de Pôster de Pesquisa Científica (PPC)

Área Temática: Patologia Oral

Resumo: Background: Platinum-based systemic therapy is used in metastatic or disseminated mucoepidermoid carcinoma (MEC), however, presented low response rates. Further, drug resistance remains a major obstacle to MEC treatment leading to tumor recurrence, disease progression, and metastasis, and this could be associated with cancer stem cells (CSCs).
Objective: Investigated the effects of SAHA (Histone deacetylase inhibitor) and Emetine (NFkB inhibitor) in CSCs derived from three MEC cell lines.
Study Design: UM-HMC-1, UM-HMC-2, and UM-HMC-3A MEC cell lines were used to investigate the effects of SAHA and Emetine on CSCs. The concentration of the drugs was obtained by a previous proliferation assay. CSCs were assayed by in vitro tumorspheres formation in two moments.
Results: First, we demonstrated that a single administration of SAHA and Emetine was able to inhibit the tumorsphere formation in all MEC cell lines. Next, we evaluated if the therapy was able to reduce the number of formed tumorspheres, and we showed a complete reduction in UM-HMC2 (** p<0.01) by Emetine, and a significant reduction in UM-HMC3a (* p<0.05) by SAHA.
Conclusion: Our findings suggest that HDAC and NFKB inhibitors might be an alternative approach to treat MEC, though they disrupt CSC.

Funding: Fapesp 2016/05710-4

Autor 1:  Paulo Victor Mendes PENAFORT

E-mail 1:  [email protected]

Autor 2:   Luan César da SILVA

E-mail 2:  [email protected]

Autor 3 :  Amanda Almeida LEITE

E-mail 3:  [email protected]

Autor 4:  Márcio Ajudarte LOPES

E-mail 4:  [email protected]

Autor 5:  Manoela Domingues MARTINS

E-mail 5:  [email protected]

Autor 6:  Pablo Agustin VARGAS

E-mail 6:  [email protected]

Autor 7:  Rogério Moraes de CASTILHO

E-mail 7:  [email protected]