NUMERO: #20220206

Título: EFFECT OF LOW-LEVEL LASER ON CYTOKINE PRODUCTION BY MONOCYTES EXPOSED TO CISPLATIN AND STIMULATED WITH P. GINGIVALIS

Nome do Apresentador: Estéfane Gomes da Silva SANTOS

Categoria do Trabalho: Trabalhos Aprovados para Disponibilização em Vídeo (ADV)

Área Temática: Estomatologia

Resumo: Objective: The aim of this study was evaluate the effect of photobiomodulation (PBM) on monocytes exposed to cisplatin and P. gingivalis (Pg). Study Design: Peripheral blood mononuclear cells from 10 healthy donors were exposed to cisplatin, irradiated with red laser (AlGaInP, 660nm, 0.028cm², 20mW, 0.71W/cm², 0.14J, 7s, 5J/cm²) and stimulated with Pg. Cell viability and cytokine expression by total monocytes and monocyte subpopulations were evaluated by flow cytometry. Results: The treatments didn´t affect the viability of monocytes and TGF-β1 expression. In Pg-stimulated cells, cisplatin reduced the expression of IL-6, IL-8, and IL-10 in total and classical monocytes and increased IL-6 and TNF expression in the intermediate monocytes. In cisplatin exposed cells, PBM reduced the expression of IL-6 and IL-8 in total, classical and intermediate monocytes and IL-10 and TNF in total monocytes. In cisplatin and Pg-stimulated cells, PBM increased the expression of IL-6 and IL-8 in total and intermediate monocytes. Conclusion: The red laser modulates the expression of cytokines by human monocytes treated with cisplatin, which may represent mechanisms of action of PBM in the management of oral mucositis.
Apoio: FAPEMIG APQ03601-16, FIP/PUC Minas 2020/24894-1S e CAPES Code 001.

Autor 1:  Estéfane Gomes da Silva SANTOS

E-mail 1:  [email protected]

Autor 2:   Fábio Fernandes Borém Bruzinga

E-mail 2:  [email protected]

Autor 3 :  Carolina Cattoni Koh

E-mail 3:  [email protected]

Autor 4:  Natália Rocha Guimarães

E-mail 4:  [email protected]

Autor 5:  Martinho Campolina Rebello Horta

E-mail 5:  [email protected]

Autor 6:  Giovanna Ribeiro Souto

E-mail 6:  [email protected]

Autor 7:  Paulo Eduardo Alencar de Souza

E-mail 7:  [email protected]