NUMERO: #20220205

Título: IMMUNOEXPRESSION OF STEM CELL MARKERS IN AMELOBLASTOMA

Nome do Apresentador: Raíssa Pinheiro de MENDONÇA

Categoria do Trabalho: Trabalhos Aprovados para Disponibilização em Vídeo (ADV)

Área Temática: Patologia Oral

Resumo: Objective: Ameloblastoma (AME) is a benign neoplasm of odontogenic origin characterized by a locally invasive growth pattern. In the interest of understand the aggressiveness of neoplasms, research on the role of stem cells has gained prominence. SOX-2, NANOG and OCT4 transcription factors are important stem cell biomarkers. Study Design: Immunohistochemistry was performed to verify the expression of these transcription factors in tissue samples of AME, dentigerous cyst (CD) and dental follicle (DF). In addition, indirect immunofluorescence and transcriptomic analysis were performed from a cell line derived from AME (AME-hTERT). Results: The expression of SOX-2, NANOG and OCT4 was observed in tissue samples and in the AME-hTERT cell line. Higher immunostaining of the proteins studied was observed in AME in comparison to CD and FD (p<0.001). Additionally, there was an association of OCT4 and SOX-2 proteins in AME neoplastic cells (p=0.0294). Transcriptomic analysis revealed a network of mutual interaction between the three transcription factors. Conclusion: The presence of these biomarkers indicates a probable role of stem cells in the genesis and progression of AME.

Autor 1:  Karolyny Martins BALBINOT

E-mail 1:  [email protected]

Autor 2:   Raíssa Pinheiro de MENDONÇA

E-mail 2:  [email protected]

Autor 3 :  Aline Marques DIAS

E-mail 3:  [email protected]

Autor 4:  Rommel Thiago Jucá RAMOS

E-mail 4:  [email protected]

Autor 5:  Maria Sueli da Silva KATAOKA

E-mail 5:  [email protected]

Autor 6:  Sérgio de Melo ALVES JÚNIOR

E-mail 6:  [email protected]

Autor 7:  João de Jesus Viana PINHEIRO

E-mail 7:  [email protected]