NUMERO: #20220180

Título: CANCER STEM CELLS IN ORAL CANCER: PHENOTYPIC COMPARISON BETWEEN SUBPOPULATIONS LABELED WITH CD44 AND CD326 ANTIBODIES.

Nome do Apresentador: Iara Gonçalves AQUINO

Categoria do Trabalho: Trabalhos Aprovados para Disponibilização em Vídeo (ADV)

Área Temática: Patologia Oral

Resumo: Objective: Cancer stem cells (CSC) comprise a subpopulation of tumor cells associated with initiation, progression, and chemoresistance. We characterized subpopulations of CSC in a metastatic cell line LN1-A derived from the oral squamous cell carcinoma (OSCC), previously isolated in our laboratory. Study design: We selected 3 subpopulations of CSC (CD44Low/CD326+, CD44+/CD326- and CD44High/CD326-) among the seven phenotypes isolated by labeling CD44 and CD326 antibodies by flow cytometry in metastatic LN-1A cells. Cell morphology, proliferation, migration, adhesion, clonogenic assay, cell cycle, cell death, and cisplatin chemoresistance were characterized. Western blotting for EFGR, FASN, p-AKT, CPT1-α, and mesenchymal epithelium transition markers were performed. Results: CD44Low/CD326+ subpopulations showed an epithelial phenotype with polygonal morphology and high E-cadherin expression, while CD44+/CD326- and CD44High/CD326- showed fusiform morphology with high Vimentin and Slug expression, suggesting a mesenchymal phenotype. Proliferation was higher in CD44+/CD326- and CD44High/CD326- cells, which was accompanied by increased levels of p-AKT. Cell adhesion and colony formation were increased in CD44+/CD326- and CD44High/CD326-, while cell death was higher in CD44Low/CD326+. No significant differences in cell cycle, cisplatin resistance, and the expression of EGFR, FASN, and, CPT1-α were observed between phenotypes. Conclusion: Our results showed that CD44+/CD326- and CD44High/CD326- cells show a more aggressive phenotype.

Autor 1:  Iara Gonçalves AQUINO

E-mail 1:  [email protected]

Autor 2:   Ana Laura Valença BIZELI

E-mail 2:  [email protected]

Autor 3 :  Florence Juana Maria CUADRA-ZELAYA

E-mail 3:  [email protected]

Autor 4:  Isadora Ferrari TEIXEIRA

E-mail 4:  [email protected]

Autor 5:  Ricardo Della COLETTA

E-mail 5:  [email protected]

Autor 6:  Débora Campanella BASTOS

E-mail 6:  [email protected]

Autor 7:  Edgard GRANER

E-mail 7:  [email protected]