NUMERO: #20220052

Título: MOLECULAR PROFILING OF TEAR FLUIDS REVEALS SIGNATURES OF MALIGNANT TRANSFORMATION INTO ORAL CANCER

Nome do Apresentador: ERISON SANTANA DOS SANTOS

Categoria do Trabalho: Apresentação Oral de Pôster de Pesquisa Científica (PPC)

Área Temática: Áreas afins no caráter multidisciplinar

Resumo: Objective: We aimed to find biological signatures of malignant transformation using non-invasive liquid biopsy. Study Design: Unstimulated tears from patients with OL (N=10), PVL (N=10), OSCC (n=14), and control patients (N=10) were collected, and submitted to discovery-based proteomics by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The data were analyzed by the software MaxQuant (proteomics), Byonics (N-glycoproteomics), and PEAKS database (post-translational modifications). Differential abundance between the groups was assessed by ANOVA following the Tukey test (p<0.05). We performed analyses of biologically processed enrichment, hierarchical clustering, and clinicopathologic correlation using Pearson correlation and linear regression (R²>0.5; p<0.05). Results: We identified a panel of 24 proteins with differential abundance between the groups that present potential capacity to predict malignant transformation. The innate immune system was the main biological process correlated with these proteins. Clinicopathologic correlations analyses showed several associations, such as epithelial dysplasia, lymph node metastasis, and clinical stages of the disease. In addition, we identified some proteins that are druggable and may be useful as therapeutic targets. Conclusion: Liquid biopsy from tear fluids may be a powerful non-invasive tool to provide insights into the process of malignant transformation into oral cancer, guiding early diagnosis and improving the prognosis.

The São Paulo Research Foundation-FAPESP (18/19922-9).

Autor 1:  ERISON SANTANA DOS SANTOS

E-mail 1:  [email protected]

Autor 2:   DANIELA CAMPOS GRANATO

E-mail 2:  [email protected]

Autor 3 :  CAROLINA MORETTO CARNIELLI

E-mail 3:  [email protected]

Autor 4:  ANA CAROLINA PRADO-RIBEIRO E SILVA

E-mail 4:  [email protected]

Autor 5:  ALAN ROGER SANTOS-SILVA

E-mail 5:  [email protected]

Autor 6:  MARCIO AJUDARTE LOPES

E-mail 6:  [email protected]

Autor 7:  ADRIANA FRANCO PAES LEME

E-mail 7:  [email protected]