NUMERO: #20260195

Título: EFFECTS OF CURCUMIN AND PHOTOBIOMODULATION THERAPY ON IRRADIATED OSTEOBLAST-LIKE CELLS: A PRELIMINARY ANALYSIS

Nome do Apresentador: Bruna BASTOS SILVEIRA

Categoria do Trabalho: Pôster - Investigação Original (IO)

Área Temática: Estomatologia

Resumo: Objective: In vitro evaluation of the effects of curcumin and photobiomodulation therapy (PBMT) on irradiated immortalized osteoblast-like human cells (SaOS-2).
Study Design: SaOS-2 cells were exposed to a single dose of ionizing radiation (16 Gray) and treated with vehicle (control), curcumin and PBMT (660 nm; 30mW; 2.09, 2.79, 4.19, and 4.88 J/cm² corresponding to irradiation times of 3, 4, 6, and 7s, respectively). After 24 hours, MTT, nitric oxide (NO), and scratch assays were performed. Data were analyzed using ANOVA and Kruskal-Wallis tests in GraphPad Prism.
Results: Curcumin at 2.5 µM and PBMT at 3, 6 and 7s showed a trend toward increased mitochondrial activity. A significant increase in NO production was also observed with 5 µM (p<0.05), while a decrease was observed at 6s (p<0.05). Additionally, 2.5 µM curcumin and PBMT at 3 and 7s enhance wound closure compared to control, without statistical significance.
Conclusion: Tested doses of curcumin and PBMT did not significantly enhance mitochondrial activity after irradiation. However, some doses showed a non-significant trend toward increased wound closure, suggesting a limited but potential effect. Future research should further investigate these therapies in irradiated osteoblasts by exploring different cell lines and optimizing culture
conditions.

Autor 1:  Bruna BASTOS SILVEIRA

E-mail 1:  [email protected]

Autor 2:   Isabella Macedo COSTA E SILVA

E-mail 2:  [email protected]

Autor 3 :  Mylene Martins Monteiro PIGHINI

E-mail 3:  [email protected]

Autor 4:  Taia Maria Berto REZENDE

E-mail 4:  [email protected]

Autor 5:  Marcelo Lazzaron LAMERS

E-mail 5:  [email protected]

Autor 6:  Manoela Domingues MARTINS

E-mail 6:  [email protected]

Autor 7:  Eliete Neves Silva GUERRA

E-mail 7:  [email protected]