NUMERO: #20260165

Título: IMMUNOHISTOCHEMICAL EXPRESSION OF CK13 AND MOLECULAR ANALYSIS OF KRT13 AND APC IN ODONTOGENIC CYSTS AND TUMORS.

Nome do Apresentador: Lucas POLTI

Categoria do Trabalho: Pôster - Investigação Original (IO)

Área Temática: Patologia Oral

Resumo: Objective: Evaluate the immunohistochemical expression of CK13 and investigate mutations in the KRT13 and APC genes in cases of calcifying odontogenic cyst (COC), dentinogenic ghost cell tumor (DGCT), adenoid ameloblastoma (AA), and conventional ameloblastoma (CA). Study design: CK13 immunohistochemical expression and specific mutations (APC E1080* and KRT13 M239V and Y281H) were analyzed in 29 cases (22 COC, 2 DGCT, 1 AA, and 4 CA). Results: CK13 expression in COC was observed in the suprabasal/superficial layers of the cystic epithelium in 14 cases-64%; ghost cells showed positivity in 12 cases-54%. DGCT cases were negative in the epithelial proliferation and positive in ghost cells. The AA case was negative. Three CA cases demonstrated positivity in suprabasal/central cells. None of the cases harbored the investigated mutations. The intronic variant KRT13 c.735+10A>G was identified in 16 COC cases, one DGCT case, one AA case, and one CA case, whereas KRT13 c.735+6C>T was detected in one COC case. Another case exhibited the previously unreported synonymous mutation KRT13 c.690G>A (p.E230E). Conclusions: CK13 expression in COC and CA suggests squamous differentiation of odontogenic epithelium. The detected KRT13 genetic variations are probably not associated with tumorigenic mechanisms in COC, DGCT, AA, and CA.
Support: CAPES, CNPq and FAPEMIG.

Autor 1:  Lucas POLTI

E-mail 1:  [email protected]

Autor 2:   Ronell BOLOGNA MOLINA

E-mail 2:  [email protected]

Autor 3 :  María Luisa PAPARELLA

E-mail 3:  [email protected]

Autor 4:  Marina GONÇALVES DINIZ

E-mail 4:  [email protected]

Autor 5:  Ricardo Santiago GOMEZ

E-mail 5:  [email protected]

Autor 6:  Silvia FERREIRA DE SOUSA

E-mail 6:  [email protected]

Autor 7:  Felipe PAIVA FONSECA

E-mail 7:  [email protected]