NUMERO: #20260090

Título: THE IMPACT OF HDAC11 ON THE IFN-ALPHA PATHWAY IN CHEMORESISTANT ORAL SQUAMOUS CELL CARCINOMA

Nome do Apresentador: Raysa Theresa Pinheiro Santos

Categoria do Trabalho: Pôster - Investigação Original (IO)

Área Temática: Oncologia

Resumo: To investigate the role of HDAC11 in oral squamous cell carcinoma (OSCC) cell lines resistant to cisplatin, focusing on the modulation of the IFN-alpha pathway and PD-L1-mediated signaling, and to determine the effects of genetic or pharmacological silencing on this pathway. OSCC cell lines CAL27 and SCC-9 (WT) were used, including cisplatin-resistant variants (CisR) and HDAC11-silenced cells (siHDAC11). In vitro experiments included siRNA-HDAC11 transfection and SAHA treatment in CisR cells, followed by gene and protein expression analyses and immunofluorescence. In vivo, CAL27 WT and CisR xenografts were established in BALB/c nude mice, and protein expression of HDAC11, IFN-alpha, IFITM1, and PD-L1 was evaluated by immunohistochemistry. HDAC11 was overexpressed in CisR cells. siRNA-HDAC11 reduced HDAC11, IFN-alpha, and IFITM1 levels, while SAHA also decreased PD-L1 expression. In vivo, CisR xenografts showed increased HDAC11 and IFN-alpha expression, which was reversed by SAHA treatment. No direct regulation of PD-L1 by HDAC11 was observed. HDAC11 may contribute to cisplatin resistance in OSCC by modulating IFN-alpha-associated pathways. Both siHDAC11 and SAHA demonstrated therapeutic potential by reducing chemoresistance markers and modulating the tumor microenvironment, suggesting a promising strategy for OSCC treatment. Funding: CAPES N°88887.905862/2023-0/CNPQ: 306040/2024-7 FAPs - FAPESP N° 2017/11780-8;2023/11261-1;2025/10664-0

Autor 1:  Raysa Theresa Pinheiro Santos

E-mail 1:  [email protected]

Autor 2:   Antonio Secco Martorano

E-mail 2:  [email protected]

Autor 3 :  Gabriela Camarneiro Siqueira

E-mail 3:  [email protected]

Autor 4:  Lucas Dias de Oliveira

E-mail 4:  [email protected]

Autor 5:  Lucas Oliveira Sousa

E-mail 5:  [email protected]

Autor 6:  Emerson de Souza Santos

E-mail 6:  [email protected]

Autor 7:  Luciana Oliveira de Almeida

E-mail 7:  [email protected]