NUMERO: #20250401

Título: THE ROLE OF MIRNA COPY NUMBER VARIATIONS IN THE PATHOGENESIS OF PLEOMORPHIC ADENOMA TO CARCINOMA EX PLEOMORPHIC ADENOMA

Nome do Apresentador: Talita de Carvalho KIMURA

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Objective: Pleomorphic adenoma (PA) is the most common benign salivary gland tumor. Despite its benign nature, some cases can recur (RPA) or progress into carcinoma ex pleomorphic adenoma (CXPA), an aggressive malignancy with poor prognosis. This study aimed to investigate copy number alterations (CNAs) in miRNA-encoding genes across PA, RPA, residual PA, and CXPA, to explore molecular differences and identify potential drivers of malignancy. Study Design: A total of 60 tumor samples were analyzed by array comparative genomic hybridization, including 13 PA, 4 RPA, 14 residual PA, and 29 CXPA. CNAs were identified using Nexus Copy Number Discovery software, and functional enrichment analysis was performed. Results and Conclusion: We identified 216 CNAs involving 2,261 miRNA genes: 117 in PA, 59 in RPA, 846 in residual PA, and 2,555 in CXPA. Chromosome 8 was frequently altered in both PA and CXPA. Six miRNA genes were recurrent across all groups, suggesting potential relevance in salivary gland tumorigenesis. Key altered miRNAs (miR-21, miR-455-3p, miR-140, miR-320a, miR-383, miR-598, and miR-486) are involved in cancer-related pathways such as glycolysis, fatty acid biosynthesis, and oncogenic signaling. These findings highlight the contribution of miRNA CNAs to tumor development and their potential as diagnostic or therapeutic targets.

Autor 1:  Talita de Carvalho KIMURA

E-mail 1:  [email protected]

Autor 2:   Luiz Paulo KOWALSKI

E-mail 2:  [email protected]

Autor 3 :  Carlos Takahiro CHONE

E-mail 3:  [email protected]

Autor 4:  Alfio José TINCANI

E-mail 4:  [email protected]

Autor 5:  Ana Cristina Victorino KREPISCHI

E-mail 5:  [email protected]

Autor 6:  Albina ALTEMANI

E-mail 6:  [email protected]

Autor 7:  Fernanda Viviane MARIANO

E-mail 7:  [email protected]