NUMERO: #20250328

Título: ORAL CANCER TREATMENT WITH SAHA AND EMETINE PLUS CISPLATIN: A PATIENT-DERIVED XENOGRAFT ANIMAL STUDY

Nome do Apresentador: Sebastião Silvério SOUSA-NETO

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Oncologia

Resumo: Objective: This in vivo study aimed to evaluate the effects of histone deacetylase inhibitors (iHDACs - SAHA) and IkBα phosphorylation (NFκB super repressor; emetine) combined with cisplatin chemotherapy in the treatment of oral cancer (OC), focusing on tumor stem cell populations, tumor growth and mice weight variation. Study Design: Patient-derived xenografts (PDXs) were established from six oral cancer patients. Fresh tumor fragments (5×5 mm) were implanted subcutaneously into 28 NOD/SCID mice (PDX-0), propagated to PDX-2, and 39 animals with tumors (200-400 mm²) were randomized into six treatment groups: control (saline or DMSO), cisplatin alone, and combinations with SAHA and/or emetine. After four weeks of intraperitoneal treatment, animals were euthanized, and organs and tumors were collected for morphological and cell cycle analysis. Results: PDX engraftment success was 57%, with latency of 60-120 days. No significant weight difference was observed among treated groups. Tumor growth control was notable in the therapy group (cisplatin + emetine + SAHA). Cell cycle analysis showed increased S-G2-M phases in groups receiving sensitizers. Some organ alterations were observed, particularly in the spleen. Conclusion:
Preliminary findings suggest that SAHA and emetine may enhance cisplatin efficacy without increasing systemic toxicity, supporting further studies with lower cisplatin doses.

Autor 1:  Sebastião Silvério SOUSA-NETO

E-mail 1:  [email protected]

Autor 2:   Brendo Vinicius Rodrigues LOUREDO

E-mail 2:  [email protected]

Autor 3 :  Luan César da SILVA

E-mail 3:  [email protected]

Autor 4:  Vivian Petersen WAGNER

E-mail 4:  [email protected]

Autor 5:  Manoela Domingues MARTINS

E-mail 5:  [email protected]

Autor 6:  Rogério Moraes CASTILHO

E-mail 6:  [email protected]

Autor 7:  Pablo Agustin VARGAS

E-mail 7:  [email protected]