NUMERO: #20250309

Título: FROM CELLS TO TUMORS: INVESTIGATING TRKB INHIBITION AS A THERAPEUTIC STRATEGY FOR ORAL SQUAMOUS CELL CARCINOMA

Nome do Apresentador: Tuany Rafaeli SCHMIDT

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Objective: To investigate the biological and therapeutic effects of selective TRK inhibitors (ANA-12, K252a, and GW) and cisplatin in oral squamous cell carcinoma (OSCC), utilizing both in vitro assays and a patient-derived xenograft (PDX) model.
Methodology: In vitro analyses were conducted using keratinocytes (HaCaT) and OSCC cell lines (Cal 27 and SCC-9) to evaluate cell viability, proliferation, migration, and cancer stem cell populations following treatment with TRK inhibitors and cisplatin. Additionally, a PDX model was established from a fresh OSCC specimen and implanted into immunodeficient BALB/c nude mice. After tumor engraftment and expansion, animals were allocated into four experimental groups: control, cisplatin, ANA-12, and combination therapy (ANA-12 plus cisplatin). Treatments were administered according to established protocols. Tumor growth was monitored weekly, and histopathological and immunohistochemical analyses were performed to assess tumor characteristics and expression of TRK, proliferation, and apoptosis markers.
Results: TRK inhibitors significantly reduced proliferation and migration in vitro. ANA-12 also decreased cancer stem cell populations in HaCaT and SCC-9 cells. In vivo, only cisplatin significantly inhibited tumor growth. TRKB expression increased across all treated groups without intergroup differences.
Conclusion: TRK inhibitors demonstrated therapeutic potential in OSCC, underscoring the relevance of molecular profiling in personalized treatment strategies.

Autor 1:  Tuany Rafaeli SCHMIDT

E-mail 1:  [email protected]

Autor 2:   Lauren Frenzel Schuch

E-mail 2:  [email protected]

Autor 3 :  Joana Schorr

E-mail 3:  [email protected]

Autor 4:  Grazielle Stelter

E-mail 4:  [email protected]

Autor 5:  Vanessa Rodrigues Velho

E-mail 5:  [email protected]

Autor 6:  Daniela Campagnol

E-mail 6:  [email protected]

Autor 7:  Manoela Domingues Martins

E-mail 7:  [email protected]