NUMERO: #20250283

Título: ANALYSIS OF PYROPTOSIS AND NECROPTOSIS INDUCED BY DYNAMIC THERAPIES IN DYSPLASTIC ORAL KERATINOCYTES: AN IN VITRO STUDY

Nome do Apresentador: Laura Barduchi de Andrade

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Objective: To investigate pyroptosis and necroptosis induced by photodynamic (PDT) and sonodynamic (SDT) therapies on dysplastic oral keratinocytes (DOK) in vitro. Methods: DOK cells were incubated with 5-aminolevulinic acid (5-ALA) for 4h in the dark, followed by exposure to LED light (630±5nm, 150mW) for 1, 3, or 5min (3, 9, or 15J/cm²). SDT was performed using 1MHz ultrasound in pulsed mode (50% duty cycle) for the same durations. For combined sono photodynamic therapy (SPDT), both sources were applied simultaneously. Cell viability was assessed by MTS, membrane rupture by LDH release, and pyroptosis and necroptosis by immunofluorescence of GSDMD and MLKL. Results: PDT showed a dose-dependent cytotoxic effect. SDT reduced viability without a clear dose-response. SPDT caused significantly greater cell death at all durations. LDH release was highest after SPDT, and immunofluorescence revealed significantly increased GSDMD and MLKL expression in SPDT-treated DOK cells. Conclusion: SPDT mediated by 5-ALA is more effective than PDT or SDT alone in inducing pyroptosis and necroptosis in dysplastic oral keratinocytes. These findings support SPDT as a promising therapeutic approach for oral epithelial dysplasia. Funding: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)/Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP-23/11402-4).

Autor 1:  Laura Barduchi de Andrade

E-mail 1:  [email protected]

Autor 2:   Sebastião Pratavieira

E-mail 2:  [email protected]

Autor 3 :  Luciana Corrêa

E-mail 3:  [email protected]

Autor 4:  Saygo Tomo

E-mail 4:  [email protected]