NUMERO: #20250262

Título: COLD ATMOSPHERIC PRESSURE PLASMA AS AN ANTI-INFLAMMATORY AGENT IN A MURINE MODEL OF CHEMOTHERAPY-INDUCED ORAL MUCOSITIS

Nome do Apresentador: Noala Vicensoto Moreira MILHAN

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Estomatologia

Resumo: Objective: To investigate the anti-inflammatory effect of cold atmospheric pressure plasma (CAP) on chemotherapy-induced oral mucositis (OM) through tissue cytokine analysis. Study design: Thirty rats received intramuscular doses of 5-fluorouracil (5-FU) for three consecutive days. On day 8, a wound was delimited in the vestibular fornix using filter paper soaked in 50% acetic acid. Half of the animals were treated with CAP at a distance of 1.5 cm for 5 minutes on the next two consecutive days, while the others were assigned as untreated controls. Euthanasia was performed on days 3, 7, and 14 after OM induction. Tissue samples were washed, macerated, and supernatants stored for immunoenzymatic assays. ELISA was used to quantify levels of TNF-α, IL-6, and IL-10. Results: The CAP-treated group showed significantly lower levels of the pro-inflammatory cytokines TNF-α and IL-6 on days 3 and 7 compared to the control group (p<0.05). Additionally, a trend toward increased levels of the anti-inflammatory cytokine IL-10 was observed, though not statistically significant (p>0.05). Conclusion: CAP demonstrated anti-inflammatory activity by reducing pro-inflammatory cytokines in a murine model of chemotherapy-induced oral mucositis. These findings suggest that CAP may represent a promising therapeutic approach for managing oral mucositis in cancer patients undergoing chemotherapy. Funding by FAPESP (São Paulo Research Foundation, 2019/25652-7; 2019/05856-7 and 2021/00046-7).

Autor 1:  Aline da Graça SAMPAIO

E-mail 1:  [email protected]

Autor 2:   Noala Vicensoto Moreira MILHAN

E-mail 2:  [email protected]

Autor 3 :  Fellype DO NASCIMENTO

E-mail 3:  [email protected]

Autor 4:  Konstantin Georgiev KOSTOV

E-mail 4:  [email protected]

Autor 5:  Cristiane Yumi KOGA-ITO

E-mail 5:  [email protected]