NUMERO: #20230929

Título: CD8+ T CELLS AND GALR2 EXPRESSION IN PBMC OF ORAL SQUAMOUS CELL CARCINOMA (OSCC) PATIENTS

Nome do Apresentador: Milena Moraes de CARVALHO

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Oncologia

Resumo: Objective: GALR2 is considered an oncogene in OSCC and CD8+ T cells are associated with anti-tumoral responses and better prognosis. The purpose was to investigate the prevalence of CD8+ T cells and GALR2 expression in peripheral blood mononuclear cells (PBMCs) of OSCC patients.
Study Design: Venous whole blood from 16 OSCC cases (n=8 T1/T2 and n=8 T3/T4) was collected at the moment of surgical resection of tumors and PBMCs were isolated. Flow cytometry was performed to identify CD3+CD8+ T cells. Prevalence of CD8+ T cells was correlated with TNM clinical staging. RT-qPCR was performed using RNA extracted from aliquots of the same PBMC samples of a subgroup of these patients (n= 6) to determine expression of GALR2.
Results: There was non-statistically significant trend of increased number of CD8+ T cells in OSCC patients with larger tumors (T3 or T4). GALR2 expression was significantly greater (p=0.005) in samples with higher number of CD8+ T cells. There was no association between tumor size and GALR2 expression in PBMC.
Conclusion: Larger OSCC tumors tend to be associated with increased number of CD8+ T cells in the peripheral blood. GALR2 expression in PBMC was greater in cases with higher numbers of CD8+ T cells.
Financial Support: FAPESP #2022/01484-0, #2020/00394-2

Autor 1:  Milena Moraes de CARVALHO

E-mail 1:  [email protected]

Autor 2:   Ianny Brum REIS

E-mail 2:  [email protected]

Autor 3 :  Camyla Rodrigues NASCIMENTO

E-mail 3:  [email protected]

Autor 4:  Danilo Paschoal FERRAREZI

E-mail 4:  [email protected]

Autor 5:  Alex de Freitas PORSANI

E-mail 5: 

Autor 6:  Flavio Ferlin ARBEX

E-mail 6:  [email protected]

Autor 7:  Carlos ROSSA JUNIOR

E-mail 7:  [email protected]